Human PEDFR/PNPLA2 Antibody (494702)

R&D Systems | Catalog # MAB5387

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 494702
View all PEDFR/PNPLA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PEDF R/PNPLA2
Val162-Thr332
Accession # Q96AD5

Specificity

Detects human PEDF R/PNPLA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PEDFR/PNPLA2 Antibody (494702)

Detection of PEDF R/PNPLA2 antibody in Y-79 Human Cell Line antibody by Flow Cytometry.

Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry.

Y-79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Monoclonal Antibody (Catalog # MAB5387, filled histogram) or isotype control anti-body (Catalog # MAB0041, open histogram), followed by Phy-coerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human PEDFR/PNPLA2 Antibody (494702)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Y‑79 human retinoblastoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human PEDF R/PNPLA2

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PEDFR/PNPLA2

PEDF R (pigment epithelium-derived factor receptor) is also called ATGL (adipose triglyceride lipase), TTS2 (transport-secretion protein 2), iPLA2 zeta (calcium-independent phospholipase A2 zeta) or desnutrin. It is a 54 kDa transmembrane protein that is member 2 of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue, where it catalyzes formation of diacyglycerol from triglyceride. It has also been found in the retina (as a receptor for PEDF), testis, cardiac and skeletal muscle. Human PEDF R shares 85% amino acid (aa) identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Entrez Gene IDs

57104 (Human); 66853 (Mouse); 361676 (Rat)

Gene Symbol

PNPLA2

UniProt

Q96AD5

Additional PEDFR/PNPLA2 Products

Product Documents for Human PEDFR/PNPLA2 Antibody (494702)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PEDFR/PNPLA2 Antibody (494702)

For research use only

Related Research Areas

Diabetes

Citations for Human PEDFR/PNPLA2 Antibody (494702)

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