Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry.
Y‑79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Monoclonal Antibody (Catalog # MAB5387, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PEDF R/PNPLA2
PEDF R (pigment epithelium-derived factor receptor) is also called ATGL (adipose triglyceride lipase), TTS2 (transport-secretion protein 2), iPLA2 zeta (calcium-independent phospholipase A2 zeta) or desnutrin. It is a 54 kDa transmembrane protein that is member 2 of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue, where it catalyzes formation of diacyglycerol from triglyceride. It has also been found in the retina (as a receptor for PEDF), testis, cardiac and skeletal muscle. Human PEDF R shares 85% amino acid (aa) identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
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