Human Pentraxin 2/SAP Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB19483-100
MAB19483-SP
Human Pentraxin 2/SAP ELISA Standard Curve.
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Human Pentraxin 2/SAP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pentraxin 2/SAP in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 910135R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pentraxin 2/SAP
His20-Val233
Accession # P02743
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pentraxin 2/SAP Monoclonal Antibody (Catalog # MAB19481).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pentraxin 2/SAP DuoSet ELISA Kit (Catalog # DY1948-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Pentraxin 2/SAP ELISA Standard Curve. Recombinant Human Pentraxin 2/SAP protein was serially diluted 2-fold and captured by Mouse Anti-Human Pentraxin 2/SAP Monoclonal Antibody (Catalog # MAB19481) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pentraxin 2/SAP Monoclonal Antibody (Catalog # MAB19483) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pentraxin 2/SAP

Pentraxin 2 (PTX2), also known as Serum Amyoid P Component (SAP), is a secreted serum glycoprotein that is a universal non-fibrillar component of amyloid deposits. These extracellular deposits of insoluble protein fibrils are the result of protein misfolding and can lead to tissue damage and disease (1, 2). PTX2 belongs to the pentraxin (pentaxin) superfamily, whose members have the characteristic pentagonal discoid arrangement of five non-covalently bound subunits. Pentraxins bind to a variety of molecules in a calcium-dependent lectin-like manner through a pattern-recognition-binding site (1, 4, 5). There are two known subfamilies of pentraxins, the classical or short pentraxin subfamily that includes the serum C-reactive Protein (CRP) and PTX2, and the fusion or long pentraxin subfamily whose members contain pentraxin-related carboxyl-terminal halves (1).

PTX2 and CRP share approximately 50% amino acid sequence identity (2, 5). They are produced and secreted by liver hepatocytes and circulates in plasma. Mouse PTX2 is a major acute-phase protein whose plasma concentrations increase dramatically during an acute phase response (2). In human where CRP is the major acute-phase protein, the plasma concentration of human PTX2 remains relatively constant in response to tissue-damage (2, 5).

PTX2 associates ubiquitously with all amyloid deposits that are implicated in a diverse range of diseases including Alzheimer’s and prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). As a non-fibrillar component, PTX2 regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. In addition to its role in the pathogenesis of amyloidoses, PTX2 also has an important physiological function in innate immunity (8).

References
  1. Goodman, A. et al. (1996) Cytokine Growth Factor Rev. 7:191.
  2. Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
  3. Hirschfield, G.M. and P.N. Hawkins (2003) Int. J. Biochem. Cell Biol. 35:1608.
  4. Emsley, J. et al. (1994) Nature 367:338.
  5. Mantzouranis, E. et al. (1985) J. Biol. Chem. 260:7752.
  6. Botto, M. et al. (1997) Nature Medicine 3:855.
  7. Pepys, M. et al. (2002) Nature 417:254.
  8. Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
Long Name
Serum Amyloid P Component
Entrez Gene IDs
325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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