Human Peroxiredoxin 1 Antibody (477609)

R&D Systems | Catalog # MAB3488

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 477609
View all Peroxiredoxin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 1
Met1-Lys199
Accession # Q06830

Specificity

Detects endogenous human Peroxiredoxin 1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Peroxiredoxin 1 Antibody (477609)

Detection of Human Peroxiredoxin 1 antibody by Western Blot.

Detection of Human Peroxiredoxin 1 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Peroxiredoxin 1 in MCF‑7 Human Cell Line.

Peroxiredoxin 1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human Peroxiredoxin 1 by Simple WesternTM.

Simple Western shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Western Blot Shows Human Peroxiredoxin 1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of Peroxiredoxin-1/PRDX1 by Immunoprecipitation.

Immunoprecipitation was performed on cell lysate of U2OS human osteosarcoma cell line using 1.0 μg of Mouse Anti-Human PRDX1 Monoclonal Antibody (Catalog # MAB3488) pre-coupled to protein G or protein A beads. Immunoprecipitated PRDX1 was detected with a Goat Anti-PRDX1 Polyclonal Antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human Peroxiredoxin 1 Antibody (477609)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Immunoprecipitation

1 µg/1 mg cell lysate
Sample: Cell lysate of U2OS human osteosarcoma cell line

Knockout Validated

Peroxiredoxin 1 is specifically detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line.

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

0.1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB3488 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxiredoxin 1

Human Peroxiredoxin1 (Prx-1, also known as Thioredoxin peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length and has two catalytic cysteines, one at Cys52 and a second at Cys173. Prx-1 is an obligate homodimer. In its inactive state, Prx-1 is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms; one shows a premature truncation after aa 171, while the second shows a deletion of aa 21‑121. Human Prx-1 is 96% aa identical to mouse Prx-1.

Alternate Names

NKEF-A, PRDX1, PRX1, TDPX2

Entrez Gene IDs

5052 (Human); 18477 (Mouse); 117254 (Rat)

Gene Symbol

PRDX1

UniProt

Q06830

Additional Peroxiredoxin 1 Products

Product Documents for Human Peroxiredoxin 1 Antibody (477609)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Peroxiredoxin 1 Antibody (477609)

For research use only

Citations for Human Peroxiredoxin 1 Antibody (477609)

Customer Reviews for Human Peroxiredoxin 1 Antibody (477609) (1)

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  • Human Peroxiredoxin 1 Antibody
    Name: Juliana Redondo
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 05/20/2016
    Used 1:1000 in 5% non fat milk, very clean blot with specific band at 22 kDa
    Human Peroxiredoxin 1 Antibody (477609) MAB3488

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