Human Peroxiredoxin 1 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human Peroxiredoxin 1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 477609
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Peroxiredoxin 1
    Met1-Lys199
    Accession # Q06830
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Peroxiredoxin 1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 1
Human Peroxiredoxin1 (Prx-1, also known as Thioredoxin peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length and has two catalytic cysteines, one at Cys52 and a second at Cys173. Prx-1 is an obligate homodimer. In its inactive state, Prx-1 is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms; one shows a premature truncation after aa 171, while the second shows a deletion of aa 21‑121. Human Prx-1 is 96% aa identical to mouse Prx-1.
  • Entrez Gene IDs:
    5052 (Human); 18477 (Mouse); 117254 (Rat)
  • Alternate Names:
    EC 1.11.1; EC 1.11.1.15; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEFA; NKEF-A; PAG; PAGAMSP23; PAGB; Peroxiredoxin 1; peroxiredoxin-1; PRDX1; proliferation-associated gene A; Proliferation-associated gene protein; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Peroxiredoxin 1 Antibody MAB3488
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Excellent
 WB Human Anonymous 05/20/2016
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Western Blot Human Peroxiredoxin 1 Antibody MAB3488
Western Blot: Human Peroxiredoxin 1 Antibody [MAB3488]

Summary

ApplicationWestern Blot
Sample TestedMesenchymal stem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsUsed 1:1000 in 5% non fat milk, very clean blot with specific band at 22 kDa

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