Human PGLYRP1/PGRP-S Antibody

Catalog # Availability Size / Price Qty
AF2590
AF2590-SP
Product Details
Citations (2)
FAQs
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Human PGLYRP1/PGRP-S Antibody Summary

Species Reactivity
Human
Specificity
Detects human PGLYRP1/PGRP-S in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human PGRP‑1 beta and recombinant mouse PGRP‑S is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PGLYRP1/PGRP-S
Gln22-Pro196
Accession # O75594
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PGLYRP1/PGRP-S (Catalog # 2590-PG)

Human PGLYRP1/PGRP-S Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human PGLYRP1/PGRP-S Biotinylated Antibody (Catalog # BAF2590)

Standard: Recombinant Human PGLYRP1/PGRP-S Protein (Catalog # 2590-PG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP1/PGRP-S

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71%, and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

References
  1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
  2. Steiner, H. (2004) Immunol. Rev. 198:83.
  3. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
  5. Dziarski, R. et al. (2003) Blood 102:689.
Long Name
Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1
Entrez Gene IDs
8993 (Human); 21946 (Mouse)
Alternate Names
peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PGLYRP; PGLYRP1; PGRPpeptidoglycan recognition protein; PGRPS; PGRP-S; PGRP-SMGC126894; PGRPSMGC126896; TAG7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L

Product Datasheets

Citations for Human PGLYRP1/PGRP-S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells
    Authors: S Downs-Cann, S Berkey, GM Delgoffe, RP Edwards, T Curiel, K Odunsi, DL Bartlett, N Obermajer
    Nat Commun, 2017;8(0):14649.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1.
    Authors: Read C, Kuijper J, Hjorth S, Heipel M, Tang X, Fleetwood A, Dantzler J, Grell S, Kastrup J, Wang C, Brandt C, Hansen A, Wagtmann N, Xu W, Stennicke V
    J Immunol, 2015;194(4):1417-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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