Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LRP-6 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human LRP-6 S1490 site.

Specificity

Detects human LRP-6 when phosphorylated at S1490 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-LRP‑6 (S1490) Antibody

Detection of Human Phospho-LRP-6 (S1490) antibody by Western Blot.

Detection of Human Phospho-LRP‑6 (S1490) by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line transfected with human LRP-6 untreated (-) or treated (+) with 100 ng/mL Recombinant Human Wnt-3a (High Purity) (Catalog # 5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-LRP‑6 (S1490) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line transfected with human LRP-6 treated with Recombinant Human Wnt‑3a (High Purity) (Catalog # 5036-WNP)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-6

LRP6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP6 associates with select Fzd multipass receptors; in the PTH system, LRP6 complexes with PTHR1. Mature human LRP6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs. Ser1490 is a key residue that impacts multiple activities. It undergoes both constitutive and receptor activation-induced phosphorylation. At least three enzymes likely act at this site, including PKA (associated with PTHR1), GSK-3 (associated with Fzd) and Cyclin Y/PFTK1 (associated with G2/M of the cell cycle). In general, Ser1490 phosphorylation results in beta -catenin stabilization, followed by either gene transcription or beta -catenin binding to centrosomes.

Long Name

LDL Receptor-related Protein 6

Alternate Names

ADCAD2, FLJ90062, FLJ90421, LRP6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Gene Symbol

LRP6

Additional LRP-6 Products

Product Documents for Human Phospho-LRP‑6 (S1490) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-LRP‑6 (S1490) Antibody

For research use only

Citations for Human Phospho-LRP‑6 (S1490) Antibody

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