Human Phospho-M-CSF R/CD115 (Y723) Antibody

Catalog #: AF3268 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3268
AF3268-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Human Phospho-M-CSF R/CD115 (Y723) by Western Blot.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-M-CSF R/CD115 (Y723) Antibody Summary

Species Reactivity

Human

Specificity

Detects human M-CSF R/CD115 when phosphorylated at Y723 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen and protein A Affinity-purified

Immunogen

Phosphopeptide containing human M-CSF R/CD115 Y723 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Phospho-M-CSF R/CD115 (Y723) by Western Blot. Western blot shows lysates of M-CSF R/CD115-transfected NS0 mouse myeloma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human M-CSF/CD115 (Catalog # 216-MC) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-M-CSF R/CD115 (Y723) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3268), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-M-CSF R/CD115 (Y723) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

View Larger

M-CSF R/CD115 in Human PBMCs. M-CSF R/CD115 phosphorylated at Y723 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rabbit Anti-Human Phospho-M-CSF R/CD115 (Y723) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF R/CD115

M-CSF R, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1‑4). M-CSF R is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF R cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF R, releasing the functional
ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References

deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
Rovida, E. et al. (2001) J. Immunol. 166:1583.
Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
Dai, X. et al. (2002) Blood 99:111.
Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
Li, M. et al. (2004) J. Neurochem. 91:623.
Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Long Name

Macrophage Colony Stimulating Factor Receptor

Entrez Gene IDs

1436 (Human); 12978 (Mouse)

Alternate Names

CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms