Detection of Human Phospho-PLC‑ gamma 2 (Y753) by Western Blot.
Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PLC‑ gamma 2 (Y753) Monoclonal Antibody (Catalog # MAB37161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC‑ gamma 2 (Y753) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 2
The Phospholipase C family consists of 13
isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2
(Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily
limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor
tyrosine kinases in response to growth factors, neurotransmitters, and
hormones, and downstream through Lck kinase-dependent phosphorylation at Y753
and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4,
5-biphosphate to produce the second messengers inositol 1, 4,
5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of
calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen
induced signaling in platelets and antigen-dependent signaling in
B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with
mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the
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