|Detection of Human Phospho-PLC‑ gamma 2 (Y753) by Western Blot. Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PLC‑ gamma 2 (Y753) Monoclonal Antibody (Catalog # MAB37161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC‑ gamma 2 (Y753) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided