Human Phospho-PYK2/FAK2 (Y402) Antibody

Catalog # Availability Size / Price Qty
MAB6210
MAB6210-SP
Detection of HumanPhospho-PYK2/FAK2 (Y402) by Western Blot.
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Product Details
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Reviews (3)

Human Phospho-PYK2/FAK2 (Y402) Antibody Summary

Species Reactivity
Human
Specificity
Detects human PYK2/FAK2 when phosphorylated at Y402.
Source
Monoclonal Mouse IgG1 Clone # 592918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human PYK2/FAK2 Y402 site
Accession # Q14289
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of HumanPhospho-PYK2/FAK2 (Y402) antibody by Western Blot. View Larger

Detection of HumanPhospho-PYK2/FAK2 (Y402) by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-PYK2/FAK2 (Y402) at approximately 105-115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-PYK2/FAK2 (Y402) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-PYK2/FAK2 (Y402) by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.2 mg/mL Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PYK2/FAK2 (Y402) at approximately 113 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PYK2/FAK2

PYK2 (Proline-rich kinase 2; also Ptk2b, Fak2, RAFTK and CAK beta ) is a 112‑116 kDa member of the Fak subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. PYK2 phosphorylation at Tyr402 is associated with enzymatic activation, intercellular localization, cell growth, cell motility, and regulating molecular associations. Over aa 390‑410, human PYK2 is 100% aa identical to mouse PYK2.

Long Name
Proline-rich Tyrosine Kinase 2
Entrez Gene IDs
2185 (Human); 19229 (Mouse); 50646 (Rat)
Alternate Names
CADTK; CADTKCAK-beta; CAK beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; EC 2.7.10; FADK2; FAK2; FAK2EC 2.7.10.2; Focal adhesion kinase 2; FRNK; PKB; ppyk2; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; PTK; PTK2B protein tyrosine kinase 2 beta; PTK2B; PYK2; PYK2Related adhesion focal tyrosine kinase; RAFTK; RAFTKFADK 2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-PYK2/FAK2 (Y402) Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
0%
4 Star
66.67%
3 Star
33.33%
2 Star
0%
1 Star
0%

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Human Phospho-PYK2/FAK2 (Y402) Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Phospho-PYK2/FAK2 (Y402) Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human Phospho-PYK2/FAK2 (Y402) Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human