Human Phospho-RelA/NFkB p65 (S536) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB72261
MAB72261-SP
Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Western Blot.
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Product Details
Citations (3)
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Human Phospho-RelA/NFkB p65 (S536) Antibody Summary

Species Reactivity
Human
Specificity
Detects human RelA/NF kappa B p65 when phosphorylated at S536.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1091B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the RelA/NF kappa B p65 S536 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-RelA/NF?B p65 (S536) antibody by Western Blot. View Larger

Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RelA/NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-RelA/NF?B p65 (S536) antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-RelA/NF kappa B p65 (S536) at approximately 66 kDa (as indicated) using 1.0 µg/mL of Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunocytochemistry Phospho-RelA/NFkB p65 (S536) antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-RelA/NFkB p65 (S536) in HT‑29 Human Cell Line. RelA/NF kappa B p65 phosphorylated at S536 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line untreated (lower panel) or treated with Calyculin A (Catalog # 1336) and Recombinant Human TNF-a (Catalog # 210-TA; upper panel) using Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RelA/NFkB p65

RelA belongs to a family of transcription factors, NF kappa B (Nuclear Factor kappa from B cells) complex, that play a fundamental role in inflammatory and immune responses. The NF kappa B complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NF kappa B1 or NF kappa B2 subunits. RelA and NF kappa B1 are the most common heterodimeric pair. The NF kappa B complex is sequestered in the cytoplasm by inhibitory I kappa B proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the I kappa B proteins allowing the NF kappa B complex to translocate to the nucleus and activate gene transcription.

Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Entrez Gene IDs
5970 (Human)
Alternate Names
MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

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Citations for Human Phospho-RelA/NFkB p65 (S536) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Isolation, structural elucidation and immuno-stimulatory properties of polysaccharides from Cuminum cyminum
    Authors: M Tabarsa, S You, K Yelithao, S Palanisamy, NM Prabhu, M Nan
    Carbohydr Polym, 2020;230(0):115636.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma
    Authors: L Gao, W Zhang, WQ Zhong, ZJ Liu, HM Li, ZL Yu, YF Zhao
    Oncol. Rep., 2018;40(5):2558-2572.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production
    Authors: R El-Amine, D Germini, VV Zakharova, T Tsfasman, EV Sheval, RAN Louzada, C Dupuy, C Bilhou-Nab, A Hamade, F Najjar, E Oksenhendl, M Lipinski, BV Chernyak, YS Vassetzky
    Redox Biol, 2017;15(0):97-108.
    Species: Human
    Sample Types: Nuclear Extract
    Applications: Western Blot

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