Human Phospho-STAT5a/b (Y694/Y699) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB41901
MAB41901-SP
Detection  of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews (2)

Human Phospho-STAT5a/b (Y694/Y699) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT5a/b when phosphorylated at Y964/Y699 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1247C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694)
Accession # P51692
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot. View Larger

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB41901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5a/b at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL‑22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Phospho-STAT5a/b (Y694/Y699) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Phospho-STAT5a/b (Y694/Y699) Antibody
By Anonymous on 04/16/2018
Application: WB Sample Tested: SK-Mel-28 human malignant melanoma cell line,SK-Mel-30 human malignant melanoma cell line,human IM9 lymphoblastoid cells Species: Human

Human Phospho-STAT5a/b (Y694/Y699) Antibody
By Anonymous on 07/24/2017
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line,MCF 10A human breast epithelial cell line Species: Human