Human Phospho-STAT5a/b (Y694/Y699) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human Phospho-STAT5a/b (Y694/Y699) Antibody Summary

Species Reactivity
Detects human STAT5a/b when phosphorylated at Y964/Y699 in direct ELISAs and Western blots.
Recombinant Monoclonal Rabbit IgG Clone # 1247C
Protein A or G purified from cell culture supernatant
Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694)
Accession # P51692
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-STAT5a/b (Y694/Y699) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB41901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5a/b at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT5a/b (Y694/Y699) antibody in IFN alpha-treated Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-STAT5a/b (Y694/Y699) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT5a/b (Y694/Y699) Molyclonal Antibody (Catalog # MAB41901) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL‑22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b

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Citation for Human Phospho-STAT5a/b (Y694/Y699) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells
    Authors: Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al.
    Pharmaceuticals (Basel)


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Human Phospho-STAT5a/b (Y694/Y699) Antibody
By Reetobrata Basu on 04/16/2018
Application: WB Sample Tested: human IM9 lymphoblastoid cells,SK-Mel-28 human malignant melanoma cell line,SK-Mel-30 human malignant melanoma cell line Species: Human

Human Phospho-STAT5a/b (Y694/Y699) Antibody
By Anonymous on 07/24/2017
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,MDA-MB-231 human breast cancer cell line Species: Human