Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PI 3-Kinase C2 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3‑Kinase C2 beta
Ser2-Ser134
Accession # O00750

Specificity

Detects human PI 3‑Kinase C2 beta in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PI 3‑Kinase C2 beta Antibody

Detection of Human PI 3-Kinase C2 beta antibody by Western Blot.

Detection of Human PI 3‑Kinase C2 beta by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PI 3-Kinase C2 beta at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PI 3-Kinase C2 beta antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

PI 3‑Kinase C2 beta in HEK293 Human Cell Line.

PI 3-Kinase C2 beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human PI 3-Kinase C2 beta by Western Blot

Detection of Human PI 3-Kinase C2 beta by Western Blot

Predator cell PIK3C2B colocalizes with PI(3)P during engulfment and is required for complete internalization.(A) CRISPR-Cas9 single-cell clone PIK3C2B knockouts of MCF-7 were screened by immunoblot. Clones indicated by asterisk were chosen for further testing. (B) Predator cell engulfment rates (left) and confluency as a measure of viability (right) for senescent MCF-7 parental cells and 3 PIK3C2B knockout clones were determined by time course imaging in a DOXO-NT culture. Underlying data can be found at S1 Data. (C) Time course live-cell imaging of a senescent MCF-7 cell expressing 2xFYVE-mCherry and PIK3C2B-GFP throughout the entire process of engulfing an NIR-MCF-7 cell, scale bar = 100 μm. (D) Left: Merged (top) and separate (middle, lower) color channels of axial planes from bottom to top of a senescent MCF-7 cell expressing 2xFYVE-mCherry and PIK3C2B-GFP, engulfing NIR-MCF-7 cells. Right, volume view reconstruction of the same image, scale bar = 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36279312), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PI 3‑Kinase C2 beta Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line

Reviewed Applications

Read 1 review rated 5 using AF7249 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PI 3-Kinase C2 beta

PIK3C2 beta (Phosphadidylinositol-4-phosphate 3 kinase C2 domain-containing beta subunit; also HsC2-PI3K) is a 175-185 kDa member of the Class II PI3/PI4 kinase family of enzymes. It is widely expressed, being found in neurons, urinary transitional and columnar epithelium, and fibroblasts. PIK3C2 beta likely participates in growth factor, integrin and chemokine signaling by interacting with select cell membrane receptors, and appears to phosphorylate both phosphatidylinositol (PI) and PI 4‑monophosphate upon activation. Human PIK3C2 beta is 1634 amino acids (aa) in length. It contains an N-terminal Pro-rich region (aa 156-174), two PI3K Class II domains (aa 622-794 and 1505-1627), a PI3K catalytic region (aa 987-1340), one PX domain (aa 1365-1481) and an NLS (aa 1555-1565). There are two potential C2 beta isoform variants. One shows a deletion of aa 850-877, while another contains a three aa insertion after Gln1016. Over aa 2-134, human PIK3C2 beta shares 91% aa identity with mouse PIK3C2 beta.

Long Name

Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-containing Subunit beta

Alternate Names

C2-PI3K, Phosphoinositide 3-kinase-C2-beta, PI 3Kinase C2 beta, PIK3C2B, PTDINS-3-kinase C2 beta

Entrez Gene IDs

5287 (Human); 240752 (Mouse); 289021 (Rat)

Gene Symbol

PIK3C2B

UniProt

O00750 (Human)

Additional PI 3-Kinase C2 beta Products

Product Documents for Human PI 3‑Kinase C2 beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PI 3‑Kinase C2 beta Antibody

For research use only

Citations for Human PI 3‑Kinase C2 beta Antibody

Customer Reviews for Human PI 3‑Kinase C2 beta Antibody (1)

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Customer Images

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  • Human PI 3-Kinase C2 beta Antibody
    Name: James Jackson
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line, MCF-7 PIK3C2B CRISPR single cell clones, U2OS human osteosarcoma cell line and MCF-7 sgPIK3C2B CRISPR Single-cell clones
    Species: Human
    Verified Customer | Posted 09/28/2020
    Human PI 3‑Kinase C2 beta Antibody AF7249

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Protocols

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail