Human PI 3-Kinase C2 beta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PI 3‑Kinase C2 beta in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PI 3‑Kinase C2 beta
    Ser2-Ser134
    Accession # O00750
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PI 3‑Kinase C2 beta by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti‑Human PI 3‑Kinase C2 beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF7249) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PI 3‑Kinase C2 beta at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry
PI 3‑Kinase C2 beta in HEK293 Human Cell Line.

PI 3‑Kinase C2 beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human PI 3‑Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase C2 beta

PIK3C2 beta (Phosphadidylinositol-4-phosphate 3 kinase C2 domain-containing beta subunit; also HsC2-PI3K) is a 175-185 kDa member of the Class II PI3/PI4 kinase family of enzymes. It is widely expressed, being found in neurons, urinary transitional and columnar epithelium, and fibroblasts. PIK3C2 beta likely participates in growth factor, integrin and chemokine signaling by interacting with select cell membrane receptors, and appears to phosphorylate both phosphatidylinositol (PI) and PI 4‑monophosphate upon activation. Human PIK3C2 beta is 1634 amino acids (aa) in length. It contains an N-terminal Pro-rich region (aa 156-174), two PI3K Class II domains (aa 622-794 and 1505-1627), a PI3K catalytic region (aa 987-1340), one PX domain (aa 1365-1481) and an NLS (aa 1555-1565). There are two potential C2 beta isoform variants. One shows a deletion of aa 850-877, while another contains a three aa insertion after Gln1016. Over aa 2-134, human PIK3C2 beta shares 91% aa identity with mouse PIK3C2 beta.

  • Long Name:
    Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-containing Subunit beta
  • Entrez Gene IDs:
    5287 (Human); 240752 (Mouse); 289021 (Rat)
  • Alternate Names:
    C2-PI3K; C2-PI3KDKFZp686G16234; EC 2.7.1; EC 2.7.1.154; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; Phosphoinositide 3-kinase-C2-beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI 3Kinase C2 beta; PI 3-Kinase C2 beta; PI3K-C2beta; PI3K-C2-beta; PIK3C2B; PTDINS-3-kinase C2 beta; PtdIns-3-kinase C2 subunit beta
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