Human PI 3-Kinase C2 beta Antibody

Catalog # Availability Size / Price Qty
Detection of Human PI 3‑Kinase C2 beta  by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (1)

Human PI 3-Kinase C2 beta Antibody Summary

Species Reactivity
Detects human PI 3‑Kinase C2 beta in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PI 3‑Kinase C2 beta
Accession # O00750
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PI 3-Kinase C2 beta  antibody by Western Blot. View Larger

Detection of Human PI 3‑Kinase C2 beta by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PI 3-Kinase C2 beta at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PI 3-Kinase C2 beta  antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

PI 3‑Kinase C2 beta in HEK293 Human Cell Line. PI 3-Kinase C2 beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase C2 beta

PIK3C2 beta (Phosphadidylinositol-4-phosphate 3 kinase C2 domain-containing beta subunit; also HsC2-PI3K) is a 175-185 kDa member of the Class II PI3/PI4 kinase family of enzymes. It is widely expressed, being found in neurons, urinary transitional and columnar epithelium, and fibroblasts. PIK3C2 beta likely participates in growth factor, integrin and chemokine signaling by interacting with select cell membrane receptors, and appears to phosphorylate both phosphatidylinositol (PI) and PI 4‑monophosphate upon activation. Human PIK3C2 beta is 1634 amino acids (aa) in length. It contains an N-terminal Pro-rich region (aa 156-174), two PI3K Class II domains (aa 622-794 and 1505-1627), a PI3K catalytic region (aa 987-1340), one PX domain (aa 1365-1481) and an NLS (aa 1555-1565). There are two potential C2 beta isoform variants. One shows a deletion of aa 850-877, while another contains a three aa insertion after Gln1016. Over aa 2-134, human PIK3C2 beta shares 91% aa identity with mouse PIK3C2 beta.

Long Name
Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-containing Subunit beta
Entrez Gene IDs
5287 (Human); 240752 (Mouse); 289021 (Rat)
Alternate Names
C2-PI3K; C2-PI3KDKFZp686G16234; EC 2.7.1; EC; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; Phosphoinositide 3-kinase-C2-beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI 3Kinase C2 beta; PI 3-Kinase C2 beta; PI3K-C2beta; PI3K-C2-beta; PIK3C2B; PTDINS-3-kinase C2 beta; PtdIns-3-kinase C2 subunit beta

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Citation for Human PI 3-Kinase C2 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Phosphoinositide species and filamentous actin formation mediate engulfment by senescent tumor cells
    Authors: WD Frey, AY Anderson, H Lee, JB Nguyen, EL Cowles, H Lu, JG Jackson
    PloS Biology, 2022;20(10):e3001858.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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Human PI 3-Kinase C2 beta Antibody
By James Jackson on 09/28/2020
Application: WB Sample Tested: MCF-7 human breast cancer cell line,MCF-7 PIK3C2B CRISPR single cell clones,MCF-7 sgPIK3C2B CRISPR Single-cell clones,U2OS human osteosarcoma cell line Species: Human