Human PIK3R4 Antibody (576514)

R&D Systems | Catalog # MAB6104

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 576514
View all PIK3R4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PIK3R4
Gln58-Arg230
Accession # Q99570

Specificity

Detects human PIK3R4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PIK3R4 Antibody (576514)

Detection of Human PIK3R4 antibody by Western Blot.

Detection of Human PIK3R4 by Western Blot.

Western blot shows lysates of TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PIK3R4 Monoclonal Antibody (Catalog # MAB6104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PIK3R4 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PIK3R4 Antibody (576514)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line

Reviewed Applications

Read 1 review rated 5 using MAB6104 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIK3R4

PIK3R4 (phosphoinositide 3-kinase regulatory subunit 4) is a 150 kDa, 1358 amino acid (aa) adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway. It is a regulatory subunit of the PI3K complex and is ubiquitously expressed. Within aa 58‑230, human PIK3R4 shares 99% aa identity with mouse and rat PIK3R4.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Alternate Names

p150, VPS15

Entrez Gene IDs

30849 (Human); 75669 (Mouse); 363131 (Rat)

Gene Symbol

PIK3R4

UniProt

Q99570

Additional PIK3R4 Products

Product Documents for Human PIK3R4 Antibody (576514)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PIK3R4 Antibody (576514)

For research use only

Customer Reviews for Human PIK3R4 Antibody (576514) (1)

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  • Human PIK3R4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 11/16/2017

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FAQs

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Associated Pathways

Akt Signaling Pathway
Akt Signaling Pathway Thumbnail