Detection of Human PIK3R4 by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PIK3R4 Monoclonal Antibody (Catalog # MAB6104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PIK3R4 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PIK3R4 (phosphoinositide 3-kinase regulatory subunit 4) is a 150 kDa, 1358 amino acid (aa) adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway. It is a regulatory subunit of the PI3K complex and is ubiquitously expressed. Within aa 58‑230, human PIK3R4 shares 99% aa identity with mouse and rat PIK3R4.
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species: Human.We have 1 review tested in 1 application: Western Blot.