Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 601214
View all PIK3R5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PIK3R5
Lys530-Pro726
Accession # Q8WYR1

Specificity

Detects human PIK3R5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PIK3R5 Antibody

Detection of Human PIK3R5 antibody by Western Blot.

Detection of Human PIK3R5 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PIK3R5 Monoclonal Antibody (Catalog # MAB6388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PIK3R5 at approximately 97 kDa and the spliced isoform at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human PIK3R5 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIK3R5

Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5; also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386. Human PIK3R5 is 86% aa identical to mouse PIK3R5. PIK3R5 is highly expressed in leukocytes, followed by spleen, lymph node, thymus, and bone marrow.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 5

Alternate Names

FOAP-2, p101-PI3K

Entrez Gene IDs

23533 (Human); 320207 (Mouse); 497931 (Rat)

Gene Symbol

PIK3R5

UniProt

Q8WYR1 (Human)

Additional PIK3R5 Products

Product Documents for Human PIK3R5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PIK3R5 Antibody

For research use only

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