Human PIK3R5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PIK3R5 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 601214
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PIK3R5
    Lys530-Pro726
    Accession # Q8WYR1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PIK3R5 by Western Blot. Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PIK3R5 Monoclonal Antibody (Catalog # MAB6388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PIK3R5 at approximately 97 kDa and the spliced isoform at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIK3R5

Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5; also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386. Human PIK3R5 is 86% aa identical to mouse PIK3R5. PIK3R5 is highly expressed in leukocytes, followed by spleen, lymph node, thymus, and bone marrow.

  • Long Name:
    Phosphoinositide-3-kinase, Regulatory Subunit 5
  • Entrez Gene IDs:
    23533 (Human); 320207 (Mouse); 497931 (Rat)
  • Alternate Names:
    F730038I15Rik; FOAP-2; p101; p101-PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit; phosphoinositide-3-kinase, regulatory subunit 5; phosphoinositide-3-kinase, regulatory subunit, polypeptide p101; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PIK3R5; PtdIns-3-kinase regulatory subunit; regulatory subunit 5, p101
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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