Human PKC theta Antibody

Catalog # Availability Size / Price Qty
MAB4368
MAB4368-SP
Human PKC theta  Antibody in Data
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Product Details
FAQs
Supplemental Products
Reviews (2)

Human PKC theta Antibody Summary

Species Reactivity
Human
Specificity
Detects human PKC theta. This antibody also detects recombinant human PKC theta, but does not detect any other recombinant human PKC family member.
Source
Monoclonal Mouse IgG2A Clone # 453416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PKC theta
His580-Ser706
Accession # Q04759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of PKC? antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PKC theta in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human PKC? Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Western Blot Detection of Human PKC? antibody by Western Blot. View Larger

Detection of Human PKC theta by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human PKC? Monoclonal Antibody (Catalog # MAB4368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC? at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Simple Western Detection of Human PKC? antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human PKC theta by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKC theta at approximately 82 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC theta Monoclonal Antibody (Catalog # MAB4368). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC theta

PKC theta (PRKCQ) is a calcium-independent phospholipid-dependent serine and threonine kinase that belongs to the novel PKC subfamily. PKC theta plays a critical role in several aspects of T cell biology, and knockout mice develop autoimmune disease. PKC theta functions through the activation of NF kappa B and AP1 transcription factors.

Long Name
Protein Kinase C theta
Entrez Gene IDs
5588 (Human); 18761 (Mouse); 85420 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.13; MGC126514; MGC141919; nPKC-theta; PKC theta; PRKCQ; PRKCT; protein kinase C theta type; protein kinase C, theta

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PKC theta Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human PKC theta Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human PKC theta Antibody
By Anonymous on 11/07/2018
Application: Antibody microarray Sample Tested: Serum and Plasma Species: Human,Mouse