Human POR/Cytochrome P450 Reductase Antibody Summary
Accession # P16435
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human POR/Cytochrome P450 Reductase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POR/Cytochrome P450 Reductase Monoclonal Antibody (Catalog # MAB6340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POR/Cytochrome P450 Reductase
NADPH-Cytochrome P450 Reductase (POR, P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). POR is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. POR is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. POR is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of POR can result in disordered steroidogenesis and Antley-Bixler syndrome.
- Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
- Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
- Flueck C.E. et al. (2004) Nat. Genet. 36:228.
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