Human POR/Cytochrome P450 Reductase Antibody

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Human POR/Cytochrome P450 Reductase Antibody in Data
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Product Details
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Human POR/Cytochrome P450 Reductase Antibody Summary

Species Reactivity
Detects human POR/Cytochrome P450 Reductase in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 739613
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human POR/Cytochrome P450 Reductase
Accession # P16435
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human POR/Cytochrome P450 Reductase antibody by Western Blot. View Larger

Detection of Human POR/Cytochrome P450 Reductase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POR/Cytochrome P450 Reductase Monoclonal Antibody (Catalog # MAB6340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (POR, P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). POR is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. POR is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. POR is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of POR can result in disordered steroidogenesis and Antley-Bixler syndrome.

  1. Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
  2. Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
  3. Flueck C.E. et al. (2004) Nat. Genet. 36:228.
Long Name
NADPH Cytochrome P450 Reductase
Entrez Gene IDs
5447 (Human)
Alternate Names
CPR; CYPOR; CYPORFLJ26468; Cytochrome P450 Reductase; DKFZp686G04235; EC; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450R; POR

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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