Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 688923
View all POT1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human POT1
Met159-Pro316
Accession # Q9NUX5

Specificity

Detects human POT1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human POT1 Antibody

Detection of Human POT1 antibody by Western Blot.

Detection of Human POT1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human POT1 Monoclonal Antibody (Catalog # MAB5299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human POT1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HL‑60 human acute promyelocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: POT1

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins.  It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes.  This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs.  Human POT1 is 634 amino acids (aa) in length.  It contains two N-terminal DNA binding OB regions (aa 1-300) and a C-terminal TPP-1 dimerization domain (aa 301-634).  There are multiple isoform variants.  There is a potential alternate start site at Met132, plus a three aa substitution for aa 43-634, a 24 aa substitution for aa 317-634, a three aa substitution for aa 457-634, and a 16 aa substitution for aa 503-634.  Over aa 159-316, human POT1 shares 71% aa identity with mouse POT1.

Long Name

Protection of Telomeres Protein 1

Alternate Names

DKFZp586D211, HPOT1, POT1 protection of telomeres 1 homolog, POT1-like telomere end-binding protein, protection of telomeres 1 homolog (S. pombe), protection of telomeres protein 1

Entrez Gene IDs

25913 (Human); 101185 (Mouse); 500054 (Rat)

Gene Symbol

POT1

UniProt

Q9NUX5

Additional POT1 Products

Product Documents for Human POT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human POT1 Antibody

For research use only

Citations for Human POT1 Antibody

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