Human PP1 alpha Catalytic Subunit Antibody

MAB6105 1 Citation Datasheet / COA / SDS

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MAB6105
MAB6105-SP

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Human PP1 alpha Catalytic Subunit Antibody in Western Blot

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PP1 alpha Catalytic Subunit Antibody Summary

Species Reactivity

Human

Specificity

Detects human PP1 alpha Catalytic Subunit in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) PP1 beta or rhPP1 gamma is observed.

Source

Monoclonal Mouse IgG_2A Clone # 490314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

synthetic peptide corresponding to Gly304-Arg317 of human PP1 alpha catalytic subunit

Gly304-Arg317
Accession # P62136

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP-100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1a Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1a, recombinant human PP1 beta, and recombinant human PP1? (5 ng/lane) were included. A specific band was detected for PP1a at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1 alpha

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. Three isoforms of the catalytic subunit are ubiquitously expressed in tissues and cell lines: PP1 alpha, PP1 beta and PP1 gamma.

Long Name

Protein Phosphatase 1 Catalytic Subunit alpha

Entrez Gene IDs

5499 (Human); 19045 (Mouse); 24668 (Rat)

Alternate Names

EC 3.1.3.16; PP1 alpha; PP1alpha; PP-1AMGC15877; PPP1A; PPP1AMGC1674; PPP1CA; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human PP1 alpha Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1