Human PP1 alpha Catalytic Subunit Antibody

• Species Reactivity
  Human
• Specificity
  Detects human PP1 alpha Catalytic Subunit in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) PP1 beta or rhPP1 gamma is observed.
• Source
  Monoclonal Mouse IgG_2A Clone # 490314
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  synthetic peptide corresponding to Gly304-Arg317 of human PP1 alpha catalytic subunit
  
  Gly304-Arg317
  Accession # P62136
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below

Western Blot click image to view larger

Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP‑100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1 alpha Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 alpha, recombinant human PP1 beta, and recombinant human PP1 gamma (5 ng/lane) were included.  A specific band was detected for PP1 alpha at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Protein Phosphatase 1 Catalytic Subunit alpha

• Entrez Gene IDs:
  5499 (Human); 19045 (Mouse); 24668 (Rat)

• Alternate Names:
  EC 3.1.3.16; PP1 alpha; PP1alpha; PP-1AMGC15877; PPP1A; PPP1AMGC1674; PPP1CA; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit

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