Human PP1 alpha Catalytic Subunit Antibody

Catalog # Availability Size / Price Qty
MAB6105
MAB6105-SP
Detection of Human PP1 alpha  by Western Blot.
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Product Details
Citations (1)
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Human PP1 alpha Catalytic Subunit Antibody Summary

Species Reactivity
Human
Specificity
Detects human PP1 alpha Catalytic Subunit in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) PP1 beta or rhPP1 gamma is observed.
Source
Monoclonal Mouse IgG2A Clone # 490314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
synthetic peptide corresponding to Gly304-Arg317 of human PP1 alpha catalytic subunit

Gly304-Arg317
Accession # P62136
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human PP1 alpha  by Western Blot. View Larger

Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP‑100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1 alpha Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 alpha, recombinant human PP1 beta, and recombinant human PP1 gamma (5 ng/lane) were included.  A specific band was detected for PP1 alpha at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1 alpha

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. Three isoforms of the catalytic subunit are ubiquitously expressed in tissues and cell lines: PP1 alpha, PP1 beta and PP1 gamma.

Long Name
Protein Phosphatase 1 Catalytic Subunit alpha
Entrez Gene IDs
5499 (Human); 19045 (Mouse); 24668 (Rat)
Alternate Names
EC 3.1.3.16; PP1 alpha; PP1alpha; PP-1AMGC15877; PPP1A; PPP1AMGC1674; PPP1CA; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Product Datasheets

Citation for Human PP1 alpha Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking
    Authors: P Cervero, C Wiesner, A Bouissou, R Poincloux, S Linder
    Nat Commun, 2018;9(1):515.
    Species: Human
    Sample Types: Protein
    Applications: WB

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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