Human PP1 alpha Catalytic Subunit Antibody Summary
Accession # P62136
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP-100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1a Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1a, recombinant human PP1 beta, and recombinant human PP1? (5 ng/lane) were included. A specific band was detected for PP1a at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP1 alpha
Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. Three isoforms of the catalytic subunit are ubiquitously expressed in tissues and cell lines: PP1 alpha, PP1 beta and PP1 gamma.
Citation for Human PP1 alpha Catalytic Subunit Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking
Authors: P Cervero, C Wiesner, A Bouissou, R Poincloux, S Linder
Nat Commun, 2018;9(1):515.
Sample Types: Protein
Applications: Western Blot
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