Human PPAR alpha /NR1C1 Antibody

Catalog # Availability Size / Price Qty
PP-H0723-00
Product Details
Citations (4)
FAQs
Supplemental Products
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Human PPAR alpha /NR1C1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human PPAR alpha and cross-reacts with mouse PPAR alpha. This antibody does not cross-react with human PPAR gamma or PPARδ. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H0723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human PPAR alpha/NR1C1
aa 4-96
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 2 μg/mL under reducingconditions with the appropriate secondary reagents to detect human PPAR alpha.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions should be determined byeach laboratory.
 
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriatesecondary reagents to detect human PPAR alpha.
 
Gel Supershift Assay
This antibody can be used at 100 μg/mL with theappropriate secondary reagents to detect human PPAR alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha ; NR1C1) is a member of the Nuclear Receptor superfamily. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three members have been identified and are called PPAR alpha, δ, and lambda. Retinoid X Receptor (RXR) is an obligate partner for PPAR.

Long Name
Peroxisome Proliferator-activated Receptor alpha
Entrez Gene IDs
5465 (Human)
Alternate Names
alpha; hPPAR; NR1C1; NR1C1MGC2237; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferator-activated receptor alpha; PPAR alpha; PPARA; PPARalpha; PPAR-alpha; PPARMGC2452

Product Datasheets

Citations for Human PPAR alpha /NR1C1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. APC-activated long non-coding RNA inhibits colorectal carcinoma pathogenesis through reducing exosome production
    Authors: FW Wang, CH Cao, K Han, YX Zhao, MY Cai, ZC Xiang, JX Zhang, JW Chen, LP Zhong, Y Huang, SF Zhou, XH Jin, XY Guan, RH Xu, D Xie
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: ChIP
  2. Activation of PPAR-alpha induces cell cycle arrest and inhibits transforming growth factor-beta1 induction of smooth muscle cell phenotype in 10T1/2 mesenchymal cells.
    Authors: Lien S, Wei S, Chang S, Chang M, Chang J, Chiu J
    Cell Signal, 2013;25(5):1252-63.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. MicroRNA-21 targets peroxisome proliferators-activated receptor-alpha in an autoregulatory loop to modulate flow-induced endothelial inflammation.
    Authors: Zhou J, Wang KC, Wu W, Subramaniam S, Shyy JY, Chiu JJ, Li JY, Chien S
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(25):10355-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. PPARalpha and PPARgamma effectively protect against HIV-induced inflammatory responses in brain endothelial cells.
    Authors: Huang W, Rha GB, Han MJ, Eum SY, Andras IE, Zhong Y, Hennig B, Toborek M
    J. Neurochem., 2008;107(2):497-509.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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