PRG-1 (plasticity-related gene-1) is a member of the lipid phosphate phosphatase-related protein (LPR) family. The 715 aa protein has a calculated (unglycosylated) molecular weight of 78 kDa. LPR members have six N-terminal transmembrane sequences and a C-terminal cytoplasmic domain thought to regulate phospholipid phosphatase activity. PRG-1 is expressed on axons in the hippocampus and is upregulated during axonal outgrowth. The C-terminal domain of human PRG-1 (amino acids 285‑715) shares 95% and 94% amino acid identity with mouse and rat PRG-1, respectively.
Human PRG‑1/LPPR4 Antibody
R&D Systems | Catalog # MAB2874
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 368701
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Product Specifications
Immunogen
E. coli-derived recombinant human PRG‑1/LPPR4
Ser285-Asp715
Accession # AAO85400
Ser285-Asp715
Accession # AAO85400
Specificity
Detects PRG‑1/LPPR4 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human PRG‑1/LPPR4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human PRG‑1/LPPR4
Sample: Recombinant Human PRG‑1/LPPR4
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PRG-1/LPPR4
Long Name
Plasticity Related Gene 1/Lipid Phosphate Phosphatase-related Protein Type 4
Alternate Names
LPPR4, PHP1, PRG1
Entrez Gene IDs
9890 (Human)
Gene Symbol
PLPPR4
UniProt
Additional PRG-1/LPPR4 Products
Product Documents for Human PRG‑1/LPPR4 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PRG‑1/LPPR4 Antibody
For research use only
Related Research Areas
Citations for Human PRG‑1/LPPR4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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