Human Pro Coagulation Factor II/
Thrombin Antibody Summary
Accession # NP_000497
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Pro Coagulation Factor II/Thrombin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pro Coagulation Factor II/Thrombin Monoclonal Antibody (Catalog # MAB1473) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Pro Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor II/Thrombin
Pro-Coagulation Factor II, also called Prothrombin, is a plasma serine protease central to the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin. Pro-Coagulation Factor II is an inactive precursor of thrombin that can be activated by proteolytic removal of the pro region.
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