Human Pro-Relaxin‑3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Pro-Relaxin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human mature Relaxin-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Pro-Relaxin-3
    Ala27-Cys142. Cross-reactivity with mature Relaxin-3 was removed.
    Accession # Q8WXF3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant human Pro-Relaxin-3
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Pro-Relaxin‑3 in Mouse Testis.

Pro-Relaxin‑3 was detected in immersion fixed frozen sections of adult mouse testis using Goat Anti-Human Pro-Relaxin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3107) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Relaxin-3

Human Relaxin-3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the insulin superfamily (1-4). Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem (3-5). The 142 amino acid (aa) Relaxin-3 pre-proprotein is processed to remove a 25 aa signal peptide and a connecting peptide (aa 53-118). The resulting mature Relaxin-3 is a 5.5 kDa, 51 aa secreted heterodimer of A (aa 119-142) and B (aa 26-52) peptides connected by two intermolecular disulfide bonds (1). Relaxin-3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated RXFP3 (4, 6). In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus (6). This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin-3 is highest (5). Relaxin-3 also binds the more widely-expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin-2 (1, 7). Although binding of Relaxin-3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor (1, 5). Relaxin-3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5 (3, 8).

  • References:
    1. Kizawa, H. et al. (2003) Regul. Pept. 113:79.
    2. Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14.
    3. Bathgate, R.A. et al. (2006) Pharmacol. Rev. 58:7.
    4. Liu, C. et al. (2005) Ann. N.Y. Acad. Sci. 1041:47.
    5. Tanaka, M. et al. (2005) Eur. J. Neurosci. 21:1659.
    6. Liu, C. et al. (2003) J. Biol. Chem. 278:50754.
    7. Rosengren, K.J. et al. (2006) J. Biol. Chem. 281:5845.
    8. Liu, C. et al. (2003) J. Biol. Chem. 278:50765.
  • Entrez Gene IDs:
    117579 (Human)
  • Alternate Names:
    H3; INSL7; M3; relaxin 3; Relaxin3; Relaxin-3; RLN3; RXN3; ZINS4
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