Human Procalcitonin Antibody

Catalog # Availability Size / Price Qty
MAB8350
MAB8350-SP
Human Procalcitonin Antibody in Western Blot
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Human Procalcitonin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Procalcitonin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 919510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Procalcitonin
Ala26-Gly117
Accession # P01258
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Procalcitonin by Western Blot. View Larger

Detection of Human Procalcitonin by Western Blot. Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB8350) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Procalcitonin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Procalcitonin in Human Thyroid. View Larger

Procalcitonin in Human Thyroid. Procalcitonin was detected in immersion fixed paraffin-embedded sections of human thyroid using Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB8350) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Procalcitonin

Human Procalcitonin (CALCA and Calcitonin) is 141 amino acids (aa) in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide and Calcitonin part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-117, human Procalcitonin shares 80% aa identity with mouse Procalcitonin. 

Entrez Gene IDs
796 (Human); 12310 (Mouse); 24241 (Rat)
Alternate Names
CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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