Human Procalcitonin (CALCA and Calcitonin) is 141 amino acids (aa) in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-38, human Procalcitonin shares 64% aa identity with mouse Procalcitonin.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Accession # P01258
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Procalcitonin Antibody
Human Procalcitonin ELISA Standard Curve.
Recombinant Human Procalcitonin protein was serially diluted 2-fold and captured by Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Procalcitonin Antibody
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Procalcitonin DuoSet ELISA Kit (Catalog # DY8350-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Procalcitonin
Alternate Names
Gene Symbol
UniProt
Additional Procalcitonin Products
Product Documents for Human Procalcitonin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Procalcitonin Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars