Human Procalcitonin Antibody

Catalog # Availability Size / Price Qty
Detection of Human Procalcitonin by Western Blot.
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Product Details
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Human Procalcitonin Antibody Summary

Species Reactivity
Detects human Procalcitonin in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 944002
Protein A or G purified from hybridoma culture supernatant
Human Procalcitonin synthetic peptide
Accession # P01258
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Procalcitonin DuoSet ELISA Kit (Catalog # DY8350-05) for convenient development of a sandwich ELISA.

5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Procalcitonin antibody by Western Blot. View Larger

Detection of Human Procalcitonin by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Procalcitonin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Procalcitonin antibody in Human Thyroid by Immunohistochemistry (IHC-P). View Larger

Procalcitonin in Human Thyroid. Procalcitonin was detected in immersion fixed paraffin-embedded sections of human thyroid using Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Procalcitonin ELISA Standard Curve. Recombinant Human Procalcitonin protein was serially diluted 2-fold and captured by Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Procalcitonin

Human Procalcitonin (CALCA and Calcitonin) is 141 amino acids (aa) in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-38, human Procalcitonin shares 64% aa identity with mouse Procalcitonin. 

Entrez Gene IDs
796 (Human); 12310 (Mouse); 24241 (Rat)
Alternate Names
CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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