Human Progranulin/PGRN Antibody

Catalog # Availability Size / Price Qty
MAB2420
MAB2420-SP
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (4)

Human Progranulin/PGRN Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Progranulin/PGRN is observed.
Source
Monoclonal Mouse IgG2A Clone # 296628
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Progranulin/PGRN
Thr18-Leu593
Accession # P28799
Formulation
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Progranulin (Catalog # 2420-PG) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Progranulin (Catalog # 2420-PG), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it does not belong to any of the well-established growth factor families (4). Human Progranulin is 593 amino acids (aa) in length and contains a 17 aa signal sequence and 5 potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VxCx5-6Cx5CCx8CCx6CCxDx2HCCPx4Cx5-6Cx2 (1). There is one alternate splice form for human Progranulin. This has a deletion of aa corresponding to aa 377‑531 of the standard form. Progranulin is secreted as a full length form (2, 4), and may undergo proteolysis leading to the release of numerous peptides made from the seven tandem repeats, called the granulins (5‑7). Human Progranulin shares 75% aa sequence identity with mouse and rat Progranulin. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), the development of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation and of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma
(4‑5, 12, 14‑19). In addition, mutations in the Progranulin gene are a cause of frontotemporal dementia, and increased expression of Progranulin is seen in activated microglia in many neurodegenerative diseases including Creutzfeldt-Jakob disease, motor neuron disease and Alzheimer's disease (20). Mutations in Progranulin causing neurodegenerative disease indicate that Progranulin is important for neuronal survival (20).

References
  1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073.
  2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863.
  3. Liu, Y. et al. (2007) BMC Cancer 7:22.
  4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347.
  5. Davidson, B. et al. (2004) Cancer 100:2139.
  6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331.
  7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993.
  8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510.
  9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727.
  10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
  11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
  12. He, Z. et al. (2003) Nat. Med. 9:225.
  13. Zhu, J. et al. (2002) Cell 111:867.
  14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
  15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
  16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
  17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
  18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
  19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.
  20. Baker, M. et al. (2006) Nature 442:916.
Entrez Gene IDs
2896 (Human); 14824 (Mouse)
Alternate Names
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

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Citations for Human Progranulin/PGRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Regulated control of gene therapies by drug-induced splicing
    Authors: AM Monteys, AA Hundley, PT Ranum, L Tecedor, A Muehlmatt, E Lim, D Lukashev, R Sivasankar, BL Davidson
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: FRET
  2. Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
    Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
    EMBO Mol Med, 2019;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  3. CSF progranulin increases in the course of Alzheimer's disease and is associated with sTREM2, neurodegeneration and cognitive decline
    Authors: M Suárez-Cal, A Capell, MÁ Araque Cab, E Morenas-Ro, K Fellerer, N Franzmeier, G Kleinberge, E Eren, Y Deming, L Piccio, CM Karch, C Cruchaga, K Paumier, RJ Bateman, AM Fagan, JC Morris, J Levin, A Danek, M Jucker, CL Masters, MN Rossor, JM Ringman, LM Shaw, JQ Trojanowsk, M Weiner, M Ewers, C Haass,,
    EMBO Mol Med, 2018;0(0):.
    Species: Human
    Sample Types: CSF
    Applications: ELISA Development (Detection)
  4. Serum biomarker for progranulin-associated frontotemporal lobar degeneration.
    Authors: Sleegers K, Brouwers N, Van Damme P, Engelborghs S, Gijselinck I, van der Zee J, Peeters K, Mattheijssens M, Cruts M, Vandenberghe R, De Deyn PP, Robberecht W, Van Broeckhoven C
    Ann. Neurol., 2009;65(5):603-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival.
    Authors: Van Damme P, Van Hoecke A, Lambrechts D, Vanacker P, Bogaert E, van Swieten J, Carmeliet P, Van Den Bosch L, Robberecht W
    J. Cell Biol., 2008;181(1):37-41.
    Species: Human
    Sample Types: CSF
    Applications: ELISA Development
  6. Missense mutations in the progranulin gene linked to frontotemporal lobar degeneration with ubiquitin-immunoreactive inclusions reduce progranulin production and secretion.
    Authors: Shankaran SS, Capell A, Hruscha AT, Fellerer K, Neumann M, Schmid B, Haass C
    J. Biol. Chem., 2007;283(3):1744-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Progranulin/PGRN Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human Progranulin/PGRN Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Progranulin/PGRN Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Progranulin/PGRN Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human Progranulin/PGRN Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human