Human Prokineticin R1/PKR1 Antibody

Catalog # Availability Size / Price Qty
MAB4655
MAB4655-SP

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Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry.
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Human Prokineticin R1/PKR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Prokineticin R1/PKR1. Stains human Prokineticin R1/PKR1 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 420849
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Prokineticin R1/PKR1
Met1-Lys393
Accession # Q8TCW9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Prokineticin R1/PKR1 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry. Human blood‑derived monocytes were stained with Human Prokineticin R1/PKR1 Monoclonal Antibody (Catalog # MAB4655, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prokineticin R1/PROKR1

Prokineticin Receptor 1 (PKR1), also called ZAQ or GPR73a (G-protein coupled receptor 73a), is a 7-transmembrane glycoprotein of the GPCR family. The extracellular portions of human PKR1 share 81% and 78% aa identity with corresponding portions of mouse PKR1 and human PKR2, with non-identity mainly in the N‑terminal sequences. Both PKR1 and PKR2 mediate the effects of prokineticins 1 and 2. This includes mitogenic regulation of angiogenesis in endocrine glands and stimulation of contraction in gastrointestinal smooth muscle.

Long Name
Prokineticin Receptor 1
Entrez Gene IDs
10887 (Human); 58182 (Mouse); 192648 (Rat)
Alternate Names
G protein-coupled receptor 73; G protein-coupled receptor ZAQ; GPR73; GPR73a; GPR73aG-protein coupled receptor 73; PKR1; PK-R1; PKR1G-protein coupled receptor ZAQ; Prokineticin R1; prokineticin receptor 1; ProkineticinR1; PROKR1; ZAQ

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