Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry.
Human blood‑derived monocytes were stained with Human Prokineticin R1/PKR1 Monoclonal Antibody (Catalog # MAB4655, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prokineticin R1/PROKR1
Prokineticin Receptor 1 (PKR1), also called ZAQ or GPR73a (G-protein coupled receptor 73a), is a 7-transmembrane glycoprotein of the GPCR family. The extracellular portions of human PKR1 share 81% and 78% aa identity with corresponding portions of mouse PKR1 and human PKR2, with non-identity mainly in the N‑terminal sequences. Both PKR1 and PKR2 mediate the effects of prokineticins 1 and 2. This includes mitogenic regulation of angiogenesis in endocrine glands and stimulation of contraction in gastrointestinal smooth muscle.
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