Human Prolactin R Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Prolactin R in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse Prolactin R and recombinant rat Prolactin R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Prolactin R
    Gln25-Asp234
    Accession # P16471
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human breast
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Prolactin R by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, MDA‑MB‑453 human breast cancer cell line, and MDA‑MB‑468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prolactin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1167) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin R at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prolactin R

The neuroendocrine pituitary hormone Prolactin (PRL), also known as lactotrophin, mamotrophin, luteotropic hormone (LTH), or luteotropin, is a secreted hormone that affects reproduction and homeostasis in vertebrates. The functions of PRL can be placed in six broad categories: 1) reproduction and lactation; 2) growth and development; 3) endocrinology and metabolism; 4) brain and behavior; 5) immunomodulation; and 6) electrolyte balance (1, 2). PRL is secreted by the anterior pituitary gland, mammary gland, placenta, brain, uterus, decidua, dermal fibroblasts, B cells, T cells, NK cells, and some breast cancer cell lines. Although the major form of PRL is a 23 kDa monomeric protein, splice variants of 14, 16, and 22 kDa have been identified. PRL has also been found to be glycosylated, phosphorylated, dimerized, and polymerized. Glycosylation, phosphorylation, dimerization, or polymerization of PRL result in lower activity (2).

Cell activation by PRL is mediated by a single chain membrane-bound protein belonging to the class 1 cytokine superfamily. The PRL receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced. Although the cytoplasmic domains of the different isoforms vary in length and composition, their extracellular domains are identical. In rats, three major PRL receptor isoforms have been described, a short (291 amino acid), an intermediate (393 amino acid), and a long (591 amino acid) (2). PRL receptors are found in mammary tissue, pituitary gland, brain, heart, lung thymus, spleen, liver, pancreas, kidney, adrenal gland, uterus, skeletal muscle, and skin (3). A soluble form of PRL-R containing the 206 NH2-terminal amino acids of the extracellular domain is secreted by mammary epithelial cells and is found in milk. Binding of the transmembrane PRL R results in ligand dimerization followed by binding and phosphorylation of Jak2. Jak2 then phosphorylates STAT and the long form of PRL R. C‑src, fyn, and the Ras/Raf/MAP kinase pathway have also been found to be activated upon PRL R ligand binding (2).

  • References:
    1. Kelly, P.A. et al. (2001) Biochem. Society Transaction 29:48.
    2. Freeman, M.E. et al. (2000) Physiol. Rev. 80:1532.
    3. Nagano, M. and P.A. Kelly (1994) J. Biol. Chem. 269:13337.
  • Long Name:
    Prolactin Receptor
  • Entrez Gene IDs:
    5618 (Human); 19116 (Mouse); 24684 (Rat)
  • Alternate Names:
    delta 4-delta 7/11 truncated prolactin receptor; delta 4-SF1b truncated prolactin receptor; hPRL receptor; hPRLrI; PRLR; PRL-R; Prolactin R; prolactin receptor delta 7/11; prolactin receptor; ProlactinR; secreted prolactin binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expression of prolactin receptors in normal canine mammary tissue, canine mammary adenomas and mammary adenocarcinomas.
    Authors: Michel E, Feldmann S, Kowalewski M, Bley C, Boos A, Guscetti F, Reichler I
    BMC Vet Res, 2012;8(0):72.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.
    Authors: Kowalewski MP, Michel E, Gram A
    Reprod. Biol. Endocrinol., 2011;9(0):109.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC
  3. Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
    Authors: Xu D, Lin L, Lin X
    Cell. Immunol., 2010;263(1):71-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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