Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PRR5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PRR5
Tyr126-Ala259
Accession # P85299

Specificity

Detects endogenous human PRR5 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PRR5 Antibody

Detection of Human PRR5 antibody by Western Blot.

Detection of Human PRR5 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human PRR5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRR5

PRR5 (Pro-rich protein 5; also Protor-1) is a 42‑46 kDa member of the Protor family of proteins. It is widely expressed, and forms part of mTORC2, an enzyme complex that regulates Atk and PKC alpha. At present, it appears PRR5 promotes mTORC2 activity. Human PRR5 is 388 amino acids (aa) in length. It possesses two RICTOR-interaction sites (aa 10‑95 and 188‑218) and a C-terminal Pro-rich region. There are multiple splice forms of PRR5. Either individually, or in-common, there is an alternate start site at Met10 ( beta isoform) and Met96 ( gamma isoform), a deletion of aa 45‑71, 161‑388, and 236‑388, a 17 aa insertion after Gly108, and a six aa substitution for aa 1‑107. Over aa 26‑259 of the 41 kDa beta isoform (NP_056181), there is 95% aa identity between human and mouse PRR5.

Long Name

Proline Rich 5 [Renal]

Alternate Names

PP610, Protor-1

Entrez Gene IDs

55615 (Human); 109270 (Mouse); 315189 (Rat)

Gene Symbol

PRR5

UniProt

P85299

Additional PRR5 Products

Product Documents for Human PRR5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PRR5 Antibody

For research use only

Related Research Areas

Autophagy

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