PRR5 (Pro-rich protein 5; also Protor-1) is a 42‑46 kDa member of the Protor family of proteins. It is widely expressed, and forms part of mTORC2, an enzyme complex that regulates Atk and PKC alpha. At present, it appears PRR5 promotes mTORC2 activity. Human PRR5 is 388 amino acids (aa) in length. It possesses two RICTOR-interaction sites (aa 10‑95 and 188‑218) and a C-terminal Pro-rich region. There are multiple splice forms of PRR5. Either individually, or in-common, there is an alternate start site at Met10 ( beta isoform) and Met96 ( gamma isoform), a deletion of aa 45‑71, 161‑388, and 236‑388, a 17 aa insertion after Gly108, and a six aa substitution for aa 1‑107. Over aa 26‑259 of the 41 kDa beta isoform (NP_056181), there is 95% aa identity between human and mouse PRR5.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Tyr126-Ala259
Accession # P85299
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human PRR5 Antibody
Detection of Human PRR5 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Applications for Human PRR5 Antibody
Western Blot
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PRR5
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PRR5 Products
Product Documents for Human PRR5 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PRR5 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
mTOR Signaling Pathway
