Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 998412
View all PTGER1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human PTGER1
Accession # P34995

Specificity

Detects human PTGER1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PTGER1 Antibody

PTGER1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

PTGER1 in Human Breast Cancer Tissue.

PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB10008) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PTGER1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB10008 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTGER1

Prostaglandin E2 Receptor 1/ PTGER1/EP1, a member of the G protein-coupled receptor family, is one of four receptors for Prostaglandin E2 (PGE2). Expression is highest in kidney and spleen, with very low expression in most other tissues. Stimulation of angiogenesis in primate ovulatory follicles by luteinizing hormone has been shown to be mediated by PGE2 through PTGER1 and PTGER2 receptors. In epidermis, E2 plays a role in keratinocyte differentiaton. Expression and function of EP1 in carcinomas has been studied. For example, cytoplasmic and nuclear localization of EP1 in breast cancer has been reported in association with prognostic markers. And activation of EP1 mediated by COX2 causes VEGFC upregulation in lung adenocarcinomas.

Long Name

Prostaglandin E2 Receptor 1, Subtype EP1

Alternate Names

EP1

Entrez Gene IDs

5731 (Human); 19216 (Mouse); 25637 (Rat)

Gene Symbol

PTGER1

UniProt

P34995 (Human)

Additional PTGER1 Products

Product Documents for Human PTGER1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTGER1 Antibody

For research use only

Customer Reviews for Human PTGER1 Antibody (1)

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  • Human PTGER1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver cancer
    Species: Human
    Verified Customer | Posted 05/31/2022
    Human PTGER1 Antibody MAB10008

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Protocols

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