PTH1R/PTHR1 in Human Kidney. PTH1R/PTHR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB57091) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PTH1R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66-68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca++-release from bone, mediated by osteoclast formation. Mature human PTH1R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1R signaling. Over aa 1-189, human PTH1R shares 88% aa identity with mouse PTH1R.
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