Human PTP1B Antibody

Catalog # Availability Size / Price Qty
AF13662
AF13662-SP
Human PTP1B Antibody in Data
2 Images
Product Details
Citations (1)
FAQs
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Human PTP1B Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PTP1B and does not cross-react with recombinant mouse PTP1B in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PTP1B
Glu2-Thr435
Accession # P18031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05-0.3 µg/mL
See below
Knockout Validated
PTP1B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PTP1B knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PTP1B antibody by Western Blot. View Larger

Detection of Human PTP1B by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human PTP1B Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human PTP1B Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PTP1B knockout HeLa cell line (KO). PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

References
  1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
  2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
  3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
  4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
  5. Elchebly, et al. (1999) Science 283:1544.
Long Name
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs
5770 (Human); 19246 (Mouse)
Alternate Names
EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1

Product Datasheets

Citation for Human PTP1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
    Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
    Thromb. Haemost., 2008;100(6):1123-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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