Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PTP1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTP1B
Glu2-Thr435
Accession # P18031

Specificity

Detects endogenous human PTP1B and does not cross-react with recombinant mouse PTP1B in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PTP1B Antibody

Detection of Human PTP1B antibody by Western Blot.

Detection of Human PTP1B by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human PTP1B Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human PTP1B Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PTP1B knockout HeLa cell line (KO). PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PTP1B Antibody

Application
Recommended Usage

Knockout Validated

PTP1B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PTP1B knockout HeLa cell line.

Western Blot

0.05-0.3 µg/mL
Sample: TF‑1 human erythroleukemic cell line and CHP‑100 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

References

  1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
  2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
  3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
  4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
  5. Elchebly, et al. (1999) Science 283:1544.

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Entrez Gene IDs

5770 (Human); 19246 (Mouse)

Gene Symbol

PTPN1

UniProt

P18031

Additional PTP1B Products

Product Documents for Human PTP1B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTP1B Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human PTP1B Antibody

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