Human PTP sigma /PTPRS Antibody

Catalog # Availability Size / Price Qty
AF3430
AF3430-SP
Detection of Human PTP sigma /PTPRS by Western Blot.
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Product Details
Citations (6)
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Human PTP sigma /PTPRS Antibody Summary

Species Reactivity
Human
Specificity
Detects the endogenous cleaved extracellular domain of human PTP sigma /PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta, PTPRδ, PTPRµ, or PTPR kappa is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PTP sigma /PTPRS
Glu30-Gly1260
Accession # NP_570924
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PTPs/PTPRS antibody by Western Blot. View Larger

Detection of Human PTP sigma /PTPRS by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PTPs/PTPRS antibody in A431 Cell Line by Immunocytochemistry (ICC). View Larger

PTP sigma /PTPRS in A431 Cell Line. PTPs/PTPRS was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human PTPs/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP sigma/PTPRS

Protein Tyrosine Phoshatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma, is an integral membrane protein with a non-glycosylated molecular weight of 214 kilodaltons. The extracellular domain, which was used as immunogen, is cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Significant levels of this protein are found in the brain and in endocrine tissues such as the pituitary gland and pancreas. Knockout mice deficient in PTPR sigma have severe neurological deficits and most die shortly after birth.

Long Name
Protein Tyrosine Phosphatase Receptor-type Sigma
Entrez Gene IDs
5802 (Human); 19280 (Mouse)
Alternate Names
EC 3.1.3.48; protein tyrosine phosphatase PTPsigma; protein tyrosine phosphatase, receptor type, S; protein tyrosine phosphatase, receptor type, sigma; PTP sigma; PTPR sigma; PTPRS; PTPSIGMA; receptor-type tyrosine-protein phosphatase S; Receptor-type tyrosine-protein phosphatase sigma; R-PTP-S; R-PTP-sigma

Product Datasheets

Citations for Human PTP sigma /PTPRS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal
    Authors: MND Svensson, M Zoccheddu, S Yang, G Nygaard, C Secchi, KM Doody, K Slowikowsk, F Mizoguchi, F Humby, R Hands, E Santelli, C Sacchetti, K Wakabayash, DJ Wu, C Barback, R Ai, W Wang, GP Sims, P Mydel, T Kasama, DL Boyle, F Galimi, D Vera, ML Tremblay, S Raychaudhu, MB Brenner, GS Firestein, C Pitzalis, AH Ekwall, SM Stanford, N Bottini
    Sci Adv, 2020;6(26):eaba4353.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder
    Authors: H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo
    Cell Rep, 2019;29(13):4362-4376.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury
    Authors: S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd
    J Neuroinflammation, 2018;15(1):90.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  5. Modulation of the proteoglycan receptor PTPsigma promotes recovery after spinal cord injury.
    Authors: Lang B, Cregg J, DePaul M, Tran A, Xu K, Dyck S, Madalena K, Brown B, Weng Y, Li S, Karimi-Abdolrezaee S, Busch S, Shen Y, Silver J
    Nature, 2015;518(7539):404-8.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  6. Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells.
    Authors: Bunin A, Sisirak V, Ghosh H, Grajkowska L, Hou Z, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, Plumas J, Hendriks W, Tremblay M, Hacker H, Staudt L, Green P, Bhagat G, Reizis B
    Immunity, 2015;43(2):277-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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