Human PXR/NR1I2 Antibody

R&D Systems | Catalog # PP-H0502-00

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Direct ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H0502
View all PXR/NR1I2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PXR/NR1I2
aa 1-78

Specificity

This antibody specifically recognizes human PXR2 but not human PXR1. The antibody epitope lies in amino acids 1-40. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human PXR/NR1I2 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect PXR2.

Western Blot

This antibody can be used at 1-10 μg/mL under reducing conditions and at 3-10 μg/mL under non-reducing conditions with the appropriate secondary reagents to detect human PXR2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when stored at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PXR/NR1I2

Pregnane X Receptor 2 (PXR2; also known as PAR2, SXR and NR1I2) is a 46 kDa member of the NR1 subfamily of the Nuclear Hormone Receptor family. It is one of at least seven splice forms from a single gene. Two basic forms exist, a 434 amino acid (aa) PXR1 form and a 473 aa PXR2 form. The PXR2 isoform has a 39 aa extension at the N-terminus and results from the use of an alternate exon 1. PXR forms heterodimers with Retinoid X Receptor (RXR) and is activated by pregnanolone, progesterone, xenobiotics, and endobiotics.

Long Name

Pregnane X Receptor

Alternate Names

BXR, NR1I2, ONR1, PAR1, PAR2, SXR

Entrez Gene IDs

8856 (Human); 18171 (Mouse)

Gene Symbol

NR1I2

Additional PXR/NR1I2 Products

Product Documents for Human PXR/NR1I2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PXR/NR1I2 Antibody

For research use only

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FAQs

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