Human R-Spondin 2 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (2)
Supplemental Products

Human R-Spondin 2 Antibody Summary

Species Reactivity
Detects human R-Spondin 2 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human (rh) R-Spondin 1, rhR-Spondin 3, and recombinant mouse R-Spondin 4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human R-Spondin 2 isoform 1
Accession # Q6UXX9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human R-Spondin 2 (Catalog # 3266-RS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 2

Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate beta -catenin signaling and overlap in expression and function (1‑3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R‑Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/ beta -catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex (3). R-Spondin 2 over-expression in Xenopus also blocks signaling of TGF-beta ligands, activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, canine, bovine, and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3, and R-Spondin 4.

  1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  2. Kim, K-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
  4. Nam, J-S. et al. (2007) Gene Expr. 281:13247.
Long Name
Roof Plate-specific Spondin 2
Entrez Gene IDs
340419 (Human); 239405 (Mouse)
Alternate Names
Cristin 2; CRISTIN2; hRspo2; MGC35555; MGC43342; Roof plate-specific spondin-2; RSPO2; R-spondin 2 homolog (Xenopus laevis); RSpondin 2; R-Spondin 2; R-spondin-2

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Citations for Human R-Spondin 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia
    Authors: R Sun, L He, H Lee, A Glinka, C Andresen, D Hübschmann, I Jeremias, K Müller-Dec, C Pabst, C Niehrs
    Cell Reports, 2021;36(7):109559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
    Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
    J. Invest. Dermatol., 2011;131(3):644-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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