Human R-Spondin 2 Antibody
Human R-Spondin 2 Antibody Summary
Accession # Q6UXX9
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 2
Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate beta -catenin signaling and overlap in expression and function (1‑3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R‑Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/ beta -catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex (3). R-Spondin 2 over-expression in Xenopus also blocks signaling of TGF-beta ligands, activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, canine, bovine, and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3, and R-Spondin 4.
- Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
- Kim, K-A. et al. (2006) Cell Cycle 5:23.
- Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
- Nam, J-S. et al. (2007) Gene Expr. 281:13247.
Citations for Human R-Spondin 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia
Authors: R Sun, L He, H Lee, A Glinka, C Andresen, D Hübschmann, I Jeremias, K Müller-Dec, C Pabst, C Niehrs
Cell Reports, 2021;36(7):109559.
Sample Types: Whole Cells
The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
J. Invest. Dermatol., 2011;131(3):644-54.
Sample Types: Cell Lysates
Applications: Western Blot
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