Human/Rat GDNF Antibody

Catalog # Availability Size / Price Qty
AB-212-NA
Neuron Survival in Response to GDNF and Neutralization by Human GDNF Antibody.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human/Rat GDNF Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat GDNF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GDNF (Catalog # 212-GD)
Recombinant Rat GDNF (Catalog # 512-GF)
Neutralization
Measured by its ability to neutralize the effect of GDNF on dorsal root ganglion neurons from E10 chick embryos. Davies, A. M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R. A. (eds): John Willey and Sons, Ltd. 95. The Neutralization Dose (ND50) is typically 2.5-5 µg/mL in the presence of 10 ng/mL Recombinant Human GDNF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Neuron Survival in Response to GDNF and Neutralization by Human GDNF Antibody. View Larger

Neuron Survival in Response to GDNF and Neutralization by Human GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human GDNF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Polyclonal Antibody (Catalog # AB‑212‑NA). The ND50 is typically 2.5-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name
Glial Cell line-derived Growth Factor
Entrez Gene IDs
2668 (Human); 25453 (Rat)
Alternate Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3

Product Datasheets

Citations for Human/Rat GDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain
    Authors: S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic
    J. Neurosci., 2018;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neut
  2. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Rat GDNF Antibody

There are currently no reviews for this product. Be the first to review Human/Rat GDNF Antibody and earn rewards!

Have you used Human/Rat GDNF Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review