Human/Rat HIF-2 alpha /EPAS1 Antibody
Human/Rat HIF-2 alpha /EPAS1 Antibody Summary
Ser543-Thr870
Accession # Q99814
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Rat HIF-2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 h. PVDF membrane was probed with 1 µg/mL of Human/Rat HIF-2a/EPAS1 Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of HIF‑2 alpha /EPAS1-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells treated with 150 µM CoCl2 overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-2a/EPAS1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2886) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Theoct-3/4promoter was detected by standard PCR.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-2 alpha/EPAS1
The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.
Product Datasheets
Citations for Human/Rat HIF-2 alpha /EPAS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Mitochondrial complex IV defects induce metabolic and signaling perturbations that expose potential vulnerabilities in HCT116 cells
Authors: O Uchenunu, AV Zhdanov, P Hutton, P Jovanovic, Y Wang, DE Andreev, L Hulea, DJ Papadopoli, D Avizonis, PV Baranov, MN Pollak, DB Papkovsky, I Topisirovi
FEBS Open Bio, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene
Authors: AS Clees, V Stolp, B Häupl, DC Fuhrmann, F Wempe, M Seibert, S Weber, A Banning, R Tikkanen, R Williams, B Brüne, H Serve, F Schnütgen, I von Metzle, N Kurrle
Cells, 2022;11(2):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
VHL Ser65 mutations enhance HIF2alpha signaling and promote epithelial-mesenchymal transition of renal cancer cells
Authors: X Ma, Z Tan, Q Zhang, K Ma, J Xiao, X Wang, Y Wang, M Zhong, Y Wang, J Li, X Zeng, W Guan, S Wang, K Gong, GH Wei, Z Wang
Cell & bioscience, 2022;12(1):52.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP -
Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia
Authors: MAS Martín-Ara, MT Rae, MG Darlison, AV Poole, JA Fraser
PLoS ONE, 2017;12(10):e0185664.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
Sci Rep, 2017;7(1):5081.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41
Nat Commun, 2016;7(0):12098.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc.
Authors: Agrawal A, Gajghate S, Smith H, Anderson DG, Albert TJ, Shapiro IM, Risbud MV
Arthritis Rheum., 2008;58(12):3798-808.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
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