Human/Rat HIF-2 alpha /EPAS1 Antibody

Catalog # Availability Size / Price Qty
AF2886
AF2886-SP
Detection of Human and Rat HIF-2 alpha /EPAS1 by Western Blot.
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Product Details
Citations (6)
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Human/Rat HIF-2 alpha /EPAS1 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat HIF‑2 alpha /EPAS1.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HIF‑2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Rat HIF-2a/EPAS1 antibody by Western Blot. View Larger

Detection of Human and Rat HIF-2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 h. PVDF membrane was probed with 1 µg/mL of Human/Rat HIF-2a/EPAS1 Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of HIF-2a/EPAS1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of HIF‑2 alpha /EPAS1-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells treated with 150 µM CoCl2 overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-2a/EPAS1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2886) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Theoct-3/4promoter was detected by standard PCR.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Long Name
Hypoxia-inducible Transcription Factor 2 alpha
Entrez Gene IDs
2034 (Human); 13819 (Mouse)
Alternate Names
Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Product Datasheets

Citations for Human/Rat HIF-2 alpha /EPAS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Mitochondrial complex IV defects induce metabolic and signaling perturbations that expose potential vulnerabilities in HCT116 cells
    Authors: O Uchenunu, AV Zhdanov, P Hutton, P Jovanovic, Y Wang, DE Andreev, L Hulea, DJ Papadopoli, D Avizonis, PV Baranov, MN Pollak, DB Papkovsky, I Topisirovi
    FEBS Open Bio, 2022;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene
    Authors: AS Clees, V Stolp, B Häupl, DC Fuhrmann, F Wempe, M Seibert, S Weber, A Banning, R Tikkanen, R Williams, B Brüne, H Serve, F Schnütgen, I von Metzle, N Kurrle
    Cells, 2022;11(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia
    Authors: MAS Martín-Ara, MT Rae, MG Darlison, AV Poole, JA Fraser
    PLoS ONE, 2017;12(10):e0185664.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
    Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
    Sci Rep, 2017;7(1):5081.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41
    Nat Commun, 2016;7(0):12098.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc.
    Authors: Agrawal A, Gajghate S, Smith H, Anderson DG, Albert TJ, Shapiro IM, Risbud MV
    Arthritis Rheum., 2008;58(12):3798-808.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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