Detection of Human/Rat IkB‑ beta by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB‑ beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific band for Human/Rat IkB‑ beta were detected at approximately 48 kDa and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human IkB‑ beta by Western Blot.
Western blot shows recombinant human Ik beta -alpha, IkB-beta, and IkB‑ epsilon (20 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB‑ beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB‑ beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NF kappa B inhibitor beta (IkB-beta ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
Entrez Gene IDs:
4793 (Human); 18036 (Mouse); 81525 (Rat)
beta; IkappaBbeta; I-kappa-B-beta; ikB-B; IkBbeta; IkB-beta; IKBBikB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; NFKBIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9ikappaBbeta
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