Human/Rat IkB-beta Antibody

(1 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat IkB-beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IkB-alpha or rhIkB-epsilon is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 347428
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IkB-beta
    Ala2-Val356
    Accession # Q15653
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Rat IkB‑ beta by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB‑ beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific band for Human/Rat IkB‑ beta were detected at approximately 48 kDa and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human IkB‑ beta  by Western Blot. Western blot shows recombinant human Ik beta -alpha, IkB-beta, and IkB‑ epsilon (20 ng/lane). PVDF mem­brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB‑ beta Mono­clonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB‑ beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-beta

NF kappa B inhibitor beta (IkB-beta ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

  • Long Name:
    I-kappa-B-beta
  • Entrez Gene IDs:
    4793 (Human); 18036 (Mouse); 81525 (Rat)
  • Alternate Names:
    beta; IkappaBbeta; I-kappa-B-beta; ikB-B; IkBbeta; IkB-beta; IKBBikB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; NFKBIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9ikappaBbeta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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