Human/Rat Tollip Antibody

Catalog # Availability Size / Price Qty
MAB4678
MAB4678-SP

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Detection of Human/Rat Tollip by Western Blot.
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Product Details
Citations (2)
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Human/Rat Tollip Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat Tollip in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 503315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Tollip
Met1-Pro274
Accession # Q9H0E2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat Tollip antibody by Western Blot. View Larger

Detection of Human/Rat Tollip by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat Tollip Monoclonal Antibody (Catalog # MAB4678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tollip at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tollip

Tollip (Toll-interacting protein) is a 28 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids(aa) in length. It contains a C2 Ca++-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa 6‑33. Human Tollip is 92% identical to mouse Tollip.

Long Name
Toll Interacting Protein
Entrez Gene IDs
54472 (Human); 54473 (Mouse); 361677 (Rat)
Alternate Names
adapter protein; FLJ33531; IL-1RAcPIP; toll interacting protein; toll-interacting protein; Tollip

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Citations for Human/Rat Tollip Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tollip-deficient zebrafish display no abnormalities in development, organ morphology or gene expression in response to lipopolysaccharide
    Authors: L Woli?ska-N, K Romaniuk, K Wojciechow, K Surga, M Kamaszewsk, H Szudrowicz, M Mi?czy?ska
    FEBS Open Bio, 2022-06-27;0(0):.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling.
    Authors: Torun A, Szymanska E, Castanon I, Wolinska-Niziol L, Bartosik A, Jastrzebski K, Mietkowska M, Gonzalez-Gaitan M, Miaczynska M
    PLoS ONE, 2015-06-25;10(6):e0130818.
    Species: Zebrafish
    Sample Types: Whole Organism
    Applications: IHC

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