Human Rev‑erb A alpha /NR1D1 Antibody

R&D Systems | Catalog # PP-A8740A-00

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Direct ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # A8740A
View all Rev-erb A alpha/NR1D1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Rev-Erb alpha /NR1D1
aa 2-130

Specificity

This antibody specifically recognizes human Rev-Erb alpha but does not recognize human Rev-Erb beta. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Rev‑erb A alpha /NR1D1 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 10 ng/mL with the appropriate secondary reagents to detect human Rev-Erb alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing and non-reducing conditions with the appropriate secondary reagents to detect human Rev-Erb alpha.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

Reverse orientation c-erbA-1 gene alpha (Rev-Erb alpha, ear1; NR1D1) is a member of the Orphan Nuclear Receptor family. Rev-Erb alpha is expressed in skeletal muscles, brown fat, liver, heart, brain, pituitary, kidney, testis, lung, and hypothalamus. Rev-Erb alpha has broad roles in cell proliferation and physiology. Rev-Erb alpha expression is regulated along a circadian rhythm.

Alternate Names

Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL

Entrez Gene IDs

9572 (Human)

Gene Symbol

NR1D1

Additional Rev-erb A alpha/NR1D1 Products

Product Documents for Human Rev‑erb A alpha /NR1D1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Rev‑erb A alpha /NR1D1 Antibody

For research use only

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