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Human RFX6 Antibody

Catalog #: MAB7780
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7780
MAB7780-SP

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Detection of Human RFX6 by Western Blot.
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Citations (1)
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Human RFX6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RFX6 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 811704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RFX6
Lys324-Thr511
Accession # Q8HWS3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RFX6 antibody by Western Blot. View Larger

Detection of Human RFX6 by Western Blot. Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RFX6 Monoclonal Antibody (Catalog # MAB7780) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RFX6 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RFX6

RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively.

Long Name
Regulatory Factor X, 6
Entrez Gene IDs
222546 (Human); 320995 (Mouse); 294395 (Rat)
Alternate Names
dJ955L16.1; DNA-binding protein RFX6; MGC33442; MTFS; Regulatory factor X 6; Regulatory factor X domain-containing protein 1; regulatory factor X, 6; RFX6; RFXDC1; RFXDC1regulatory factor X domain containing 1

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Citation for Human RFX6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Calcineurin/NFATc2 and PI3K/AKT signaling maintains beta -cell identity and function during metabolic and inflammatory stress
    Authors: Carly M. Darden, Srividya Vasu, Jordan Mattke, Yang Liu, Christopher J. Rhodes, Bashoo Naziruddin et al.
    iScience

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