Human ROR gamma /RORC/NR1F3 Antibody

Catalog #: PP-H6437-00 Datasheet / COA / SDS
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Human ROR gamma /RORC/NR1F3 Antibody Summary

Species Reactivity
This antibody specifically recognizes human ROR gamma and does not cross-react with human ROR alpha or human ROR beta. Not yet tested in other species.
Monoclonal Mouse IgG2A Clone # H6437
Protein A or G purified from hybridoma culture supernatant
Recombinant human ROR gamma/RORC/NR1F3
A liquid formulation in physiologic saline with 0.1% NaN3.

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Recommended Concentration
Western Blot
This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human ROR gamma.
Optimal dilutions should be determined by each laboratory.
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human ROR gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the Orphan Nuclear Receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extension. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name
Retinoic Acid-Related Orphan Receptor gamma
Entrez Gene IDs
6097 (Human)
Alternate Names
MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-Related Orphan Nuclear Receptor Variant 2; RAR-related orphan receptor C; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR gamma; RORC; RORG; RORGMGC129539; RZRG; RZRGRZR-GAMMA; TOR

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Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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