Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 672816
View all S100A11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A11
Ala2-Thr105
Accession # P31949

Specificity

Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A7, A8, A9, A10, A13, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S100A11 Antibody

Detection of Human S100A11 antibody by Western Blot.

Detection of Human S100A11 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human S100A11 Monoclonal Antibody (Catalog # MAB4874) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human S100A11 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A11

S100A11 (also S100C and calgizzarin) is a 10-12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins.  It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth.  Human S100A11 is 105 amino acids (aa) in length.  It contains two EF-hand motifs (aa 13-49 and 55-90) and one high-affinity Ca-binding site (aa 68-79), and binds annexin I with its C-terminal half.  There may be one alternative splice form that apparently contains a series of mutations over aa 61-79 and shows 90% overall aa identity to the standard form.  Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.

Long Name

S100 Calcium Binding Protein A11

Alternate Names

Calgizzarin, MLN70, S100C

Entrez Gene IDs

6282 (Human); 20195 (Mouse); 445415 (Rat)

Gene Symbol

S100A11

UniProt

P31949

Additional S100A11 Products

Product Documents for Human S100A11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A11 Antibody

For research use only

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