|Detection of Human S100A11 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4874) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
S100A11 (also S100C and calgizzarin) is a 10‑12 kDa member of the S100 family, EF-hand superfamily of Ca‑binding proteins. It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth. Human S100A11 is 105 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑49 and 55‑90) and one high-affinity Ca‑binding site (aa 68‑79), and binds annexin I with its C-terminal half. There may be one alternative splice form that apparently contains a series of mutations over aa 61‑79 and shows 90% overall aa identity to the standard form. Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.
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