Human S100A8 Antibody Summary
Accession # P05109
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human S100A8 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A8 in Human Lung Cancer Tissue. S100A8 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.
Citations for Human S100A8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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S100A8/A9 Is a Marker for the Release of Neutrophil Extracellular Traps and Induces Neutrophil Activation
Authors: EGG Sprenkeler, J Zandstra, ND van Kleef, I Goetschalc, B Verstegen, CEM Aarts, H Janssen, ATJ Tool, G van Mierlo, R van Brugge, I Jongerius, TW Kuijpers
Sample Types: Whole Cells
Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes
Authors: K Asleh, GL Negri, SE Spencer Mi, S Colborne, CS Hughes, XQ Wang, D Gao, CB Gilks, SKL Chia, TO Nielsen, GB Morin
Nature Communications, 2022;13(1):896.
Sample Types: Whole Tissue
Lipid and protein tumor markers for head and neck squamous cell carcinoma identified by imaging mass spectrometry
Authors: J Schmidt, B Kajtár, K Juhász, M Péter, T Járai, A Burián, L Kereskai, I Gerlinger, T Tornóczki, G Balogh, L Vígh, L Márk, Z Balogi
Sample Types: Whole Tissue
Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma
Authors: KC Chang, RY Chen, YC Wang, LY Hung, LJ Medeiros, YP Chen, TY Chen, JC Yang, PM Chiang
Scientific Reports, 2020;10(1):21342.
Sample Types: Cell Lysates
Applications: Western Blot
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