Human S100A8 Antibody

Catalog # Availability Size / Price Qty
MAB4570
MAB4570-SP
Detection of Human S100A8 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human S100A8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A8 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) S100A8/A9 is observed and no cross-reactivity with rhS100A9, recombinant mouse (rm) S100A8, rmS100A9, or rmS100A8/A9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 749916
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human S100A8
Met1-Glu93
Accession # P05109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human S100A8 antibody by Western Blot. View Larger

Detection of Human S100A8 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry S100A8 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

S100A8 in Human Lung Cancer Tissue. S100A8 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A8

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name
S100 Calcium Binding Protein A8
Entrez Gene IDs
6279 (Human); 20201 (Mouse)
Alternate Names
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

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Citations for Human S100A8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. S100A8/A9 Is a Marker for the Release of Neutrophil Extracellular Traps and Induces Neutrophil Activation
    Authors: EGG Sprenkeler, J Zandstra, ND van Kleef, I Goetschalc, B Verstegen, CEM Aarts, H Janssen, ATJ Tool, G van Mierlo, R van Brugge, I Jongerius, TW Kuijpers
    Cells, 2022;11(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes
    Authors: K Asleh, GL Negri, SE Spencer Mi, S Colborne, CS Hughes, XQ Wang, D Gao, CB Gilks, SKL Chia, TO Nielsen, GB Morin
    Nature Communications, 2022;13(1):896.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Lipid and protein tumor markers for head and neck squamous cell carcinoma identified by imaging mass spectrometry
    Authors: J Schmidt, B Kajtár, K Juhász, M Péter, T Járai, A Burián, L Kereskai, I Gerlinger, T Tornóczki, G Balogh, L Vígh, L Márk, Z Balogi
    Oncotarget, 2020;11(28):2702-2717.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma
    Authors: KC Chang, RY Chen, YC Wang, LY Hung, LJ Medeiros, YP Chen, TY Chen, JC Yang, PM Chiang
    Scientific Reports, 2020;10(1):21342.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human S100A8 Antibody
By Anonymous on 10/25/2021
Application: IHC Sample Tested: Appendix Species: Human