Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 472806
View all S100B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271

Specificity

Detects human S100B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) S100A1, 2, 6, 7, 10, 11, 13, 16, rmS100A4, 8, 9, or rhS100P.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human S100B Antibody

Detection of Human S100B antibody by Western Blot.

Detection of Human S100B by Western Blot.

Western blot shows lysate of human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100B at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human S100B antibody by Simple WesternTM.

Detection of Human S100B by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human S100B Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

2 µg/mL
Sample: Human brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Alternate Names

beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta

Entrez Gene IDs

6285 (Human)

Gene Symbol

S100B

UniProt

P04271

Additional S100B Products

Product Documents for Human S100B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100B Antibody

For research use only

Citations for Human S100B Antibody

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