Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 778518
View all Secretagogin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Secretagogin
Asn164-Pro276
Accession # O76038

Specificity

Detects human Secretagogin in ELISAs and Western Blots. Detects mouse Secretagogin in Western Blots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Secretagogin Antibody

Detection of Human and Mouse Secretagogin antibody by Western Blot.

Detection of Human and Mouse Secretagogin by Western Blot.

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Secretagogin antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Secretagogin in Human Pancreas.

Secretagogin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse Secretagogin antibody by Simple WesternTM.

Detection of Human and Mouse Secretagogin by Simple WesternTM.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for Secretagogin at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Secretagogin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue

Western Blot

1 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue

Reviewed Applications

Read 1 review rated 5 using MAB4878 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Secretagogin

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Alternate Names

CALBL, SCGN, SECRET, SEGN, Setagin

Entrez Gene IDs

10590 (Human); 214189 (Mouse); 306942 (Rat)

Gene Symbol

SCGN

UniProt

O76038 (Human)

Additional Secretagogin Products

Product Documents for Human Secretagogin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Secretagogin Antibody

For research use only

Customer Reviews for Human Secretagogin Antibody (1)

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  • Human Secretagogin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 04/05/2022
    human pancreas tissue staining
    Incubated tissue overnight at 4C at 15 µg/mL
    Human Secretagogin Antibody MAB4878

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Protocols

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