Serpin A12 (serine proteinase inhibitor-clade A#12; also known as vaspin) is a 45‑50 kDa secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral adipocytes, and adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown. Human Serpin A12 is 414 amino acids (aa) in length. It contains a 20 aa signal sequence and a 394 aa mature region. A potential "active" inhibitor site lies between Arg363 and Met378. Mature human Serpin A12 shares 61% aa identity with mouse Serpin A12.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 484223
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A12
Leu20-Lys414
Accession # Q8IW75
Leu20-Lys414
Accession # Q8IW75
Specificity
Detects human Serpin A12 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A1, A3, A4, A5, A6, B8, 9, C1, D1, E1, F2, G1, I1, I2, recombinant mouse (rm) Serpin E2, F1, recombinant rat Serpin B6, rhA1 Antitrypsin, rhAngiotensinogin, or rmPEDF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Serpin A12 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A12, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A12, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Serpin A12
Sample: Recombinant Human Serpin A12
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin A12
Alternate Names
OL-64, Vaspin
Gene Symbol
SERPINA12
UniProt
Additional Serpin A12 Products
Product Documents for Human Serpin A12 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin A12 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
