Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 373008
View all Serpin D1/Heparin Cofactor II Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Arg498
Accession # P05546

Specificity

Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human Serpin A3, A4, B6, B8, C1, I1, I2, recombinant mouse Serpin A1, A6, D1, E1, E2, F1, or F2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Serpin D1/Heparin Cofactor II Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)

Reviewed Applications

Read 4 reviews rated 3.8 using MAB3198 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin D1/Heparin Cofactor II

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.

Alternate Names

HC2, HCF2, Heparin Cofactor II, LS2

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Gene Symbol

SERPIND1

UniProt

P05546

Additional Serpin D1/Heparin Cofactor II Products

Product Documents for Human Serpin D1/Heparin Cofactor II Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin D1/Heparin Cofactor II Antibody

For research use only

Customer Reviews for Human Serpin D1/Heparin Cofactor II Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Serpin D1/Heparin Cofactor II Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Serpin D1/Heparin Cofactor II Antibody MAB3198
  • Human Serpin D1/Heparin Cofactor II Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human Serpin D1/Heparin Cofactor II Antibody
    Name: Ed Lo
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Human Serpin D1/Heparin Cofactor II Antibody
    Name: Carly Garrison
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/09/2018

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail