Human SF-1/NR5A1 Antibody

Catalog # Availability Size / Price Qty
MAB10724-100
MAB10724-SP
SF‑1/NR5A1 in HepG2 Human Cell Line.
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Human SF-1/NR5A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SF‑1/NR5A1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1036706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human SF‑1/NR5A1 synthetic peptide
Accession # Q13285
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human testis
Immunocytochemistry
8-25 µg/mL
Immersion fixed HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

SF‑1/NR5A1 in HepG2 Human Cell Line. SF‑1/NR5A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative control) using Mouse Anti-Human SF‑1/NR5A1 Monoclonal Antibody (Catalog # MAB10724) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

SF‑1/NR5A1 in Human Testis. SF‑1/NR5A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human SF‑1/NR5A1 Monoclonal Antibody (Catalog # MAB10724) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in sperm cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF-1/NR5A1

NR5A1 (STF1) is a transcriptional activator essential for sexual differentiation and formation of the primary steroidogenic tissues.  Mutations in the NR5A1 gene result in multiple diseases ranging from infertility (SFPG8), premature ovarian failure (POF7), adrenal insufficiency (AINR), female to male sex reversal (SRXX4) and male to female sex reversal (SRXY3).

Long Name
Steroidogenic Factor 1
Entrez Gene IDs
2516 (Human)
Alternate Names
AD4BP; AD4BPSTF-1; Adrenal 4-binding protein; ELP; FTZ1adrenal 4 binding protein; FTZF1; FTZF1nuclear receptor AdBP4; Fushi tarazu factor homolog 1; NR5A1; Nuclear receptor subfamily 5 group A member 1; nuclear receptor subfamily 5, group A, member 1; SF1; SF-1; SF-1POF7; SF1steroidogenic factor 1; Steroid hormone receptor Ad4BP; steroidogenic factor-1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human SF-1/NR5A1 Antibody

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Human SF-1/NR5A1 Antibody
By Anonymous on 03/02/2022
Application: ICC/IF Sample Tested: Human adrenocortical carcinoma (HAC) cells Species: Human