Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SHBG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SHBG
Leu30-His402
Accession # P04278

Specificity

Detects human SHBG in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SHBG Antibody

SHBG antibody in Human Liver by Immunohistochemistry (IHC-P).

SHBG in Human Liver.

SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2656) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human SHBG by Western Blot.

Western blot shows lysates of Human Liver. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SHBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2656) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SHBG at approximately ~37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of SHBG by Immunohistochemistry

Detection of SHBG by Immunohistochemistry

Immunohistochemistry of SHBG expression in prostate cancer tissues.(A) Positive and negative control of SHBG in liver tissues. (B) Weak SHBG positivity is shown in a benign prostate tumor and strong SHBG immunoreactivity is revealed in a malignant Gleason score 8 cancer tissue. (C) Representative immunohistochemical images of different scores of prostate cancer samples are shown. Bar scale in all of the images is 150 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23936228), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SHBG Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: Human Liver

Reviewed Applications

Read 2 reviews rated 5 using AF2656 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SHBG

Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Long Name

Sex Hormone-binding Globulin

Alternate Names

ABP

Entrez Gene IDs

6462 (Human)

Gene Symbol

SHBG

UniProt

P04278

Additional SHBG Products

Product Documents for Human SHBG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SHBG Antibody

For research use only

Citations for Human SHBG Antibody

Customer Reviews for Human SHBG Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human SHBG Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human SHBG Antibody AF2656
  • Human SHBG Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line
    Species: Human
    Verified Customer | Posted 10/28/2018
    Human SHBG Antibody AF2656

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Protocols

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